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method of screening plant extract with diuretic potential

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method of screening plant extract with diuretic potential ...

The anti- inflammatory activity was found out by Carragenan induced paw edema method by using standard , (S-II, S-III) 100gms of dried plant powder was extracted with chloroform and ethyl acetate to obtain a brown , Screening of Anti-Inflammatory activity ,

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PRELIMINARY PHYTOCHEMICAL SCREENING OF SIX MEDICINAL ...

Methods: Screening of six selected medicinal plants was performed for the presence of tannins, flavonoids, ... Preliminary phytochemical tests for plant extracts Phytoconstituents Test Observation Tannins ... reduces appetite and behaves as diuretic [23]. It

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Biological screening of various medicinal plant extracts ...

All extracts were sterilized by filtering through a 0.22-µm filter (Millex®), and sterile filter paper disks (gal ss microfiber fitlers, Whatman ®; 6 mm in diameter) were impregnated with 13 µL of extract. There were 5 replicates in each plate and 2 plates for each extract

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Phytochemical screening and in vitro bioactivity of ...

Phytochemical screening for major constituents was undertaken us-ing standard qualitative methods as described by Sofowora (1993) method. The test for tannins was carried out by subjecting 3 g of each plant extract in 6 ml of distilled water, filtered and ferric chlo-ride reagents added to the filtrate. For cardiac glycosides, Killer-

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SCREENING METHODS IN THE STUDY OF ANTIMICROBIAL

protozoan’s and helminthes are used for screening of plant extracts. Evaluation of the performance of a susceptibility test should have ease to use, reproducibility, test sensitivity and specificity (Struelens et al., 1995). AST standard tests can be conveniently divided into diffusion and dilution methods. Common

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method of screening plant extract with diuretic potential

evaluation of the diuretic activity of the aqueous and 80 thus it is presumed to be advantageous to ‘pretreat’ or ‘prime’ the test animals with various fluids in screening agents for potential diuretic activity the aqueous and 80 methanol extracts of the leaves of ajuga remota showed an increase in urine volume that appeared to vary with dose and time as well as the nature of the extract

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Evaluation of antioxidant and diuretic activities of ...

In the present study, the diuretic and antioxidant potential of Clitoria ternatea leaf extracts were evaluated. Preliminary phytochemical screening of three solvent extracts [methanol (MCTL), chloroform, and aqueous) of the leaves revealed that the extracts were rich in alkaloids, glycosides, carbohydrates, flavonoids, and saponins. Among the various

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Diuretic potential of various extracts of Oxystelma ...

The petroleum ether extract was found to possess the most effective diuretic activity, thereby supporting the traditional claim of the plant as a diuretic. Phytochemical screening of this extract...

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Evaluation of Diuretic Potential of Aqueous Root Extracts ...

Background: Tecoma stans a medicinal plant is used for its various pharmacological potential use to treat various ailments. Objective: To evaluate the diuretic potential of aqueous extracts of Tecomastans roots in normal rats after acute and sub-chronic oral administration. Materials and methods: Aqueous extracts of Tecomastans(200 and 400 mg/kg) along with the reference drug, furosemide (10 ...

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PRELIMINARY PHYTOCHEMICAL SCREENING OF SIX MEDICINAL ...

Terpenoids 2ml extract + 2ml (CH 3CO)2O + 2-3 drops conc. H2SO 4 Deep red coloration Saponins (Foam Test) (a) 5ml extract + 5ml H 2O + heat Froth appears (b) 5ml extract + Olive oil (few drops) Emulsion forms Steroids (Salkowski Test) 2ml extract + 2ml CHCl3 + 2ml H 2SO 4 (conc.) Reddish brown ring at the junction Phlobatannins (Precipitate Test)

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Phytochemical Screening and Analysis of Selected

Froth test for saponins glycosides: By using distilled water the plant extract is diluted and this was shaken for 15 minutes in graduated cylinder. The presence of saponins was indicated by the formation of 1 cm layer of foam.

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Phytochemical screening by FTIR spectroscopic analysis of ...

Phytochemical screening by FTIR spectroscopic analysis of leaf extracts ... IR spectroscopy method. ... Dried powder of different solvent extracts of each plant materials were used for FTIR analysis. 10 mg of the dried extract powder was encapsulated in 100 mg of .

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Plants from Puerto Rico with anti-Mycobacterium ...

Method: In Phase I of this study, ethanolic leaf extracts of fifty local plants were submitted to preliminary screening to assess their in vitro Mycobacterium smegmatis inhibitory activity using the Bauer-Kirby disk diffusion method. In Phase II, the definitive screening of the six most promising extracts which inhibited M. smegmatis were assayed for their MTB inhibitory activity using the BACTEC 460 susceptibility test

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Avoiding pitfalls in determining antimicrobial activity

22-05-2019  Using resazurin as a measure of growth. The need for a different method to examine plant extracts was identified and discussed by two papers published in 1988. Mann and Markham [ 12] worked on essential oils and applied resazurin, a redox indicator that is used in the dairy industry.

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method of screening plant extract with diuretic potential

evaluation of the diuretic activity of the aqueous and 80 thus it is presumed to be advantageous to ‘pretreat’ or ‘prime’ the test animals with various fluids in screening agents for potential diuretic activity the aqueous and 80 methanol extracts of the leaves of ajuga remota showed an increase in urine volume that appeared to vary with dose and time as well as the nature of the extract

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Diuretic activity of different extracts of Biophytum ...

Preliminary phytochemical screening of the extracts revealed the presence of alkaloids, carbohydrates, flavonoids, tannins, and phenolic compounds. Therefore, some of these components may have played role in the observed diuretic activity profile.

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Screening natural product extracts for ... - Plant Methods

06-01-2021  Therefore, enzyme inhibition assays targeting α-glucosidase, α-amylase and lipase are widespread in research, and screening plant extracts and natural products for inhibitory activity against these enzymes is a common approach for the discovery of potential antidiabetic and antiobesity drugs to treat and manages these metabolic diseases [18,19,20].

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Evaluation of the diuretic activity of ethanolic and ...

(40°C) to yield ethanolic and aqueous extract, respectively. Preliminary phytochemical screening of TD. The preliminary phytochemical investigation was carried out with ethanolic and aqueous extracts of leaves of TD for the qualitative identification of phytochemical constituents. Phytochemical tests were carried out by standard methods [16-17].

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In-vitro qualitative and quantitative analysis of certain ...

Materials and Methods Chemical compounds having diuretic and antioxidant property of plants and or plant origin were screened for their in vitro diuretic and antioxidant potential. Screening was done on the basis of available scientific literature and indigenous technical knowledge of common people. Bio-organics of plant origin were

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Evaluation of Diuretic Potential of Aqueous Root Extracts ...

Background: Tecoma stans a medicinal plant is used for its various pharmacological potential use to treat various ailments. Objective: To evaluate the diuretic potential of aqueous extracts of Tecomastans roots in normal rats after acute and sub-chronic oral administration. Materials and methods: Aqueous extracts of Tecomastans(200 and 400 mg/kg) along with the reference drug, furosemide (10 ...

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Phytochemical screening by FTIR spectroscopic analysis of ...

Phytochemical screening by FTIR spectroscopic analysis of leaf extracts ... IR spectroscopy method. ... Dried powder of different solvent extracts of each plant materials were used for FTIR analysis. 10 mg of the dried extract powder was encapsulated in 100 mg of .

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Phytochemical Screening and Analysis of Selected

Froth test for saponins glycosides: By using distilled water the plant extract is diluted and this was shaken for 15 minutes in graduated cylinder. The presence of saponins was indicated by

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Antimicrobial potentials of medicinal plant’s extract

01-11-2019  The MIC values of plant extracts were found to exhibit significant at 0.35–0.80 mg/mL. Among the tested plants, Albizia lebbeck, Cleistanthus collinus, Emblica officinalis, Eucalyptus deglupta, Eupatorium odoratum, Oxalis corniculata and Hevea brasiliensis were reported to show the minimum MIC values of 0.35–0.60 mg/mL.

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Development of a method to screen and isolate potential ...

Development of a method to screen and isolate potential xanthine oxidase inhibitors from Panax japlcus var via ultrafiltration liquid chromatography combined with counter-current chromatography. Li S(1), Tang Y(2), Liu C(3), Li J(4), Guo L(5), Zhang Y(2).

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